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Registro Completo |
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Biblioteca(s): |
Embrapa Semiárido. |
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Data corrente: |
18/07/2022 |
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Data da última atualização: |
20/04/2023 |
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Tipo da produção científica: |
Artigo em Periódico Indexado |
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Autoria: |
SILVA, C. M. de J.; DIAS, R. de C. S.; SANTOS, J. S. dos; SOUZA, F. de F.; MELO, N. F. de. |
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Afiliação: |
CARLA MARIA DE JESUS SILVA, Universidade Estadual de Feira de Santana, Feira de Santana, BA; RITA DE CASSIA SOUZA DIAS, CPATSA; JOICE SIMONE DOS SANTOS, Instituto Federal do Sertão Pernambucano, Campus Ouricuri, PE; FLAVIO DE FRANCA SOUZA, CPATSA; NATONIEL FRANKLIN DE MELO, CPATSA. |
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Título: |
Induction of polyploidy in watermelon genotype with powdery mildew resistance (Podosphaera xanthii). |
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Ano de publicação: |
2022 |
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Fonte/Imprenta: |
Revista Caatinga, v. 35, n. 3, p. 505-513, 2022. |
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DOI: |
http://dx.doi.org/10.1590/1983-21252022v35n301rc |
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Idioma: |
Inglês |
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Conteúdo: |
Triploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew?resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant. MenosTriploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew?resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics... Mostrar Tudo |
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Palavras-Chave: |
Duplicação cromossômica; Melancia triploide; Podosphaera xanthii; Tetraploidia. |
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Thesagro: |
Citogenética; Citogenética Vegetal; Citrullus Lanatus; Doença; Melancia; Oídio. |
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Thesaurus Nal: |
Cytogenetics; Tetraploidy; Watermelons. |
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Categoria do assunto: |
G Melhoramento Genético |
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URL: |
https://ainfo.cnptia.embrapa.br/digital/bitstream/doc/1144783/1/INDUCTION-OF-POLYPLOIDY-IN-WATERMELON-2022.pdf
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Marc: |
LEADER 02593naa a2200337 a 4500
001 2144783
005 2023-04-20
008 2022 bl uuuu u00u1 u #d
024 7 $ahttp://dx.doi.org/10.1590/1983-21252022v35n301rc$2DOI
100 1 $aSILVA, C. M. de J.
245 $aInduction of polyploidy in watermelon genotype with powdery mildew resistance (Podosphaera xanthii).$h[electronic resource]
260 $c2022
520 $aTriploid watermelon is highly appreciated by the most demanding markets, and due to its small size, it is ideal for consumption by small families. With the growth in areas cultivated with seedless watermelon worldwide, there is a demand for the development of tetraploid germplasm to obtain triploid hybrids with better agronomic performance. This study performed two tests to induce polyploidy in the powdery mildew?resistant line developed by Embrapa Semi-Arid, LDRO, under different colchicine concentrations and application methods. In Experiment 1, the seeds were treated with colchicine (0.0%, 0.1%, and 0.2%) for 24 h and 48 h. In Experiment 2, 0.2% colchicine was applied by different methods: (a) directly on the seed (MDS) with and without scarification, (b) on seeds with radicle emission (MER), (c) at the insertion point between the hypocotyl and the root (MIHR), (d) at the seedling apex (MAP), and (e) in the inverted hypocotyl (MHI). Chromosome count (cytogenetic analysis), number of chloroplasts per pair of guard cells, number of stomata, seedling height, and hypocotyl diameter were measured. In the LDRO line, chromosomal duplication occurred in some plant cells, but it was not possible to obtain 100% tetraploid plants. Colchicine (0.2%) for 48 h without mechanical scarification induced chromosomal duplication in watermelon. The analysis of the number of chloroplasts identified the level of ploidy early, reducing the number of plants needed to be evaluated by cytogenetics, which allowed us to more accurately identify the different levels of ploidy of the plant.
650 $aCytogenetics
650 $aTetraploidy
650 $aWatermelons
650 $aCitogenética
650 $aCitogenética Vegetal
650 $aCitrullus Lanatus
650 $aDoença
650 $aMelancia
650 $aOídio
653 $aDuplicação cromossômica
653 $aMelancia triploide
653 $aPodosphaera xanthii
653 $aTetraploidia
700 1 $aDIAS, R. de C. S.
700 1 $aSANTOS, J. S. dos
700 1 $aSOUZA, F. de F.
700 1 $aMELO, N. F. de
773 $tRevista Caatinga$gv. 35, n. 3, p. 505-513, 2022.
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Registro original: |
Embrapa Semiárido (CPATSA) |
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 | Acesso ao texto completo restrito à biblioteca da Embrapa Clima Temperado. Para informações adicionais entre em contato com cpact.biblioteca@embrapa.br. |
Registro Completo
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Biblioteca(s): |
Embrapa Clima Temperado. |
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Data corrente: |
02/03/2010 |
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Data da última atualização: |
17/05/2022 |
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Tipo da produção científica: |
Resumo em Anais de Congresso |
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Autoria: |
MOURA, G. C. de; PEREIRA, M. C.; CASTILHOS, P. M.; VIZZOTTO, M.; ANTUNES, L. E. C. |
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Afiliação: |
G. C. de. MOURA; M. C. PEREIRA; P. M. CASTILHOS; MARCIA VIZZOTTO, CPACT; LUIS EDUARDO CORREA ANTUNES, CPACT. |
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Título: |
Caracterização funcional de mirtilos ´climax' provinientes de plantas com diferentes coberturas do solo. |
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Ano de publicação: |
2009 |
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Fonte/Imprenta: |
In: ENCONTRO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 11., 2009, Fraiburgo. Anais... Caçador: Epagri, 2009. v. 2 Resumos, p. 69. |
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Idioma: |
Português |
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Palavras-Chave: |
Climax. |
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Thesagro: |
Mirtilo. |
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Categoria do assunto: |
-- |
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Marc: |
LEADER 00627naa a2200181 a 4500
001 1747170
005 2022-05-17
008 2009 bl uuuu u00u1 u #d
100 1 $aMOURA, G. C. de
245 $aCaracterização funcional de mirtilos ´climax' provinientes de plantas com diferentes coberturas do solo.
260 $c2009
650 $aMirtilo
653 $aClimax
700 1 $aPEREIRA, M. C.
700 1 $aCASTILHOS, P. M.
700 1 $aVIZZOTTO, M.
700 1 $aANTUNES, L. E. C.
773 $tIn: ENCONTRO NACIONAL SOBRE FRUTICULTURA DE CLIMA TEMPERADO, 11., 2009, Fraiburgo. Anais... Caçador: Epagri, 2009.$gv. 2 Resumos, p. 69.
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